How to avoid pitfalls in antibody use

How to avoid pitfalls in antibody use

Antibody use is ubiquitous in the biomedical sciences. However, determining best research practices has not been trivial. Many commercially available antibodies and antibody-conjugates are poorly characterized and lack proper validation. Uncritical application of such useless tools has contributed to the reproducibility crisis in biomedical research. Despite early initiatives such as MIAPAR or ...

Pitfalls in compressed sensing reconstruction and how to avoid them

Multidimensional NMR can provide unmatched spectral resolution, which is crucial when dealing with samples of biological macromolecules. The resolution, however, comes at the high price of long experimental time. Non-uniform sampling (NUS) of the evolution time domain allows to suppress this limitation by sampling only a small fraction of the data, but requires sophisticated algorithms to recon...

Molecular Dynamics with Multiple Time Scales: How to Avoid Pitfalls.

Multiple time scale methodologies have gained widespread use in molecular dynamics simulations and are implemented in a variety of ways across numerous packages. However, performance of the algorithms depends upon the details of the implementation. This is particularly important in the way in which the nonbonded interactions are partitioned. In this work, we show why some previous implementatio...

Consultant appraisal: pitfalls and how to avoid them.

Appraisal for consultants offers a unique opportunity to discuss and record professional issues, identify constraints to practice and plan personal development. Participating in appraisal also offers a painless route to revalidation. Making the best use of the process requires preparation and planning on the part of the appraiser and the appraisee. This article discusses some of the potential p...

Legal pitfalls to avoid in criminal interrogations.